| Biological Information | |
|---|---|
| Background Information: | The cAMP Hunter™ Follitropin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Follitropin Alfa based activation of FSHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FSHR, a naturally Gs coupled GPCR. Upon activation of FSHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This assay has been optimized and qualified with Gonal-F (not supplied in the bioassay kit). Gonal-F is a registered trademark of Merck KGAA. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | Glycoprotein Hormone Receptors |
| Sub Family: | Class A |
| Accession Number: | NM_000145 |
| Target Name: | FSHR |
| Target Aliases: | Follicle stimulating hormone receptor, LGR1, ODG1, FSHR1, FSHRO |
| Target Species: | Human |
| Cell Background: | CHO-K1 |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | cAMP Detection |
| Detection Method: | Chemiluminescence |
| Bioassay Data | |
| Qualified With: | Gonal-f® |
| Trademark Statement: | Gonal-f® is a registered trademark of Merck KGAA |
| Clinical Relevance | |
| Therapeutic Area: | Metabolic Diseases |
| Additional Information | |
| Brand: | cAMP Hunter™ |
cAMP Hunter™ Follitropin Alfa Bioassay Kit
The cAMP Hunter™ Follitropin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Follitropin Alfa based activation of FSHR GPCR activity via detection of levels of intracellular cAMP.
View bioassay qualification data.
User Manuals & Protocols
70-392 cAMP Hunter Follitropin Alfa Bioassay Kit REV0 User Manual
View DocumentDatasheets
95-0119Y2x Datasheet
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