cAMP Hunter™ Follitropin Alfa Bioassay Kit

Background Information:

The cAMP Hunter™ Follitropin Alfa Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Follitropin Alfa based activation of FSHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type FSHR, a naturally Gs coupled GPCR. Upon activation of FSHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This assay has been optimized and qualified with Gonal-F (not supplied in the bioassay kit). Gonal-F is a registered trademark of Merck KGAA. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

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Target Class:

GPCR

Family:

Glycoprotein Hormone

Sub Family:

Class A

Protein Name:

FSH

Protein Aliases:

Follitropin receptor

Accession Number:

NM_000145

UniProt Number:

P23945

Gene Name:

FSHR

Gene ID:

2492

Gene Aliases:

FSHRO|LGR1

Target Species:

Human

Cell Background:

CHO-K1