cAMP Hunter™ eXpress OXER1 CHO-K1 GPCR Assay

Background Information:

The cAMP Hunter™ OXER1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 5-oxo-ETE) based activation of OXER1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type OXER1, a naturally Gi/Go coupled GPCR. Upon activation of OXER1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Leukotriene

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

OXE

Protein Aliases:

G-protein coupled receptor 170|G-protein coupled receptor R527|G-protein coupled receptor TG1019

Accession Number:

NM_148962.4

UniProt Number:

Q8TDS5

Gene Name:

OXER1

Gene ID:

165140

Gene Aliases:

GPCR|TG1019|GPR170

Target Species:

Human

Cell Background:

CHO-K1