cAMP Hunter™ eXpress NPFFR1 CHO-K1 GPCR Assay

Background Information:

The cAMP Hunter™ NPFFR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. neuropeptide FF, RFRP-3) based activation of NPFFR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type NPFFR1, a naturally Gi/Go coupled GPCR. Upon activation of NPFFR1, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Neuropeptide FF / Neuropeptide AF

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

NPFF1

Protein Aliases:

RFamide-related peptide receptor OT7T022

Accession Number:

NM_022146

UniProt Number:

Q9GZQ6

Gene Name:

NPFFR1

Gene ID:

64106

Gene Aliases:

GPR147|NPFF1

Target Species:

Human

Cell Background:

CHO-K1