cAMP Hunter™ eXpress GCGR CHO-K1 GPCR Assay

Cells were plated in a 96-well plate and stimulated with a control agonist (+ Forskolin for Gi targets). Following stimulation, signal was detected according to the recommended protocol.

The cAMP Hunter™ eXpress GCGR CHO-K1 GPCR Assay measures GCGR (GPCR) activity via increased levels of intracellular cAMP. eXpress kits contain all assay materials: cells, reagents, and plates.

Catalog #: 95-0042E2CP2L

$1,789.00 – $5,989.00

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Biological Information
Background Information:
The cAMP Hunter™ GCGR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. glucagon) based activation of GCGR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GCGR, a naturally Gs coupled GPCR. Upon activation of GCGR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
GPCR
Family:
Glucagon Receptor Family
Sub Family:
Class B
Coupling:
Gs
Accession Number:
NM_000160
Target Name:
GCGR
Target Aliases:
Glucagon receptor, GGR, GL-R, MVAH
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
cAMP Detection
Detection Method:
Chemiluminescence
Additional Information
Brand:
cAMP Hunter™

User Manuals & Protocols

70-234 cAMP Hunter eXpress GPCR Assay REV10 User Manual

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Datasheets

95-0042E2_cAMP Hunter_eXpress GCGR CHO-K1 GPCR Assay

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