Biological Information
Background Information:
The cAMP Hunter™ PTHR1 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. parathyroid hormone) based activation of PTHR1 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR1 , a naturally Gs coupled GPCR. Upon activation of PTHR1 , the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Parathyroid Hormone
Sub Family:
Class B
Protein Name:
PTH1
Protein Aliases:
PTH/PTHr receptor|Parathyroid hormone 1 receptor|PTH1 receptor
Accession Number:
AY449732
UniProt Number:
Q03431
Gene Name:
PTH1R
Gene ID:
5745
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
cAMP Detection
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
cAMP Hunter™