cAMP Hunter™ CHO-K1 NPFFR1 Gi Cell Line

Background Information:

The cAMP Hunter™ NPFFR1 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. neuropeptide FF, RFRP-3) based activation of NPFFR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type NPFFR1 , a naturally Gi/Go coupled GPCR. Upon activation of NPFFR1 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Neuropeptide FF / Neuropeptide AF

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

NPFF1

Protein Aliases:

RFamide-related peptide receptor OT7T022

Accession Number:

NM_022146

UniProt Number:

Q9GZQ6

Gene Name:

NPFFR1

Gene ID:

64106

Gene Aliases:

GPR147|NPFF1

Target Species:

Human

Cell Background:

CHO-K1