cAMP Hunter™ CHO-K1 LTB4R Gi Cell Line

The cAMP Hunter™ CHO-K1 LTB4R Gi Cell Line measures LTB4R (GPCR) activity via increased levels of intracellular cAMP. This cell line ships as two vials of cryopreserved cells.

Catalog #: 95-0085C2 Size: 2 vials
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Biological Information
Background Information:
The cAMP Hunter™ LTB4R Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. leukotriene B4) based activation of LTB4R GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type LTB4R , a naturally Gi/Go coupled GPCR. Upon activation of LTB4R , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:
GPCR
Family:
Leukotriene Receptors
Sub Family:
Class A
Coupling:
Gi/Go & Gq
Accession Number:
AY322535
Target Name:
LTB4R
Target Aliases:
Leukotriene B4 receptor, BLT1, BLTR, CMKRL1, GPR16, LTBR1, P2RY7, P2Y7, LTB4R
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
cAMP Detection
Detection Method:
Chemiluminescence
Additional Information
Brand:
cAMP Hunter™

User Manuals & Protocols

70-233 cAMP Hunter Gs and Gi Cell Lines REV10 User Manual

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Datasheets

95-0085C2 Datasheet

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