cAMP Hunter™ CHO-K1 GPR81 Gi Cell Line

Background Information:

The cAMP Hunter™ GPR81 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. L-lactate) based activation of GPR81 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR81 , a naturally Gi/Go coupled GPCR. Upon activation of GPR81 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Hydroxycarboxylic Acid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

GPR81

Protein Aliases:

G-protein coupled receptor 81|HCA1

Accession Number:

NM_032554

UniProt Number:

Q9BXC0

Gene Name:

HCAR1

Gene ID:

27198

Gene Aliases:

GPR104|GPR81|HCA1|FKSG80

Target Species:

Human

Cell Background:

CHO-K1