| Biological Information | |
|---|---|
| Background Information: | The cAMP Hunter™ CHO-K1 GPBAR1 Bioassay Kit provides a robust and highly sensitive functional, cell-based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. TGR5 receptor agonist) based activation of GLP-1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP-1R, a naturally Gs coupled GPCR. Upon activation of GLP-1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. |
| Target Class: | GPCR |
| Family: | Bile Acid Receptor (GPBA) |
| Accession Number: | NM_001077194.1 |
| Target Name: | GPBAR1 |
| Target Aliases: | G protein-coupled bile acid receptor 1, BG37, TGR5, M-BAR, GPCR19, GPR131 |
| Target Species: | Human |
| Cell Background: | CHO-K1 |
| Usage | |
| Product Type: | Bioassay Kits |
| Application: | Potency; Commercial Release & Stability |
| Kit Components: |  |
| Storage Conditions: | Store in vapor phase of liquid nitrogen. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Type: | Functional |
| Assay Measures: | cAMP Detection |
| Detection Method: | Chemiluminescence |
| Additional Information | |
| Brand: | cAMP Hunter™ |
cAMP Hunter™ CHO-K1 GPBAR1 Bioassay Kit
The cAMP Hunter™ CHO-K1 GPBAR1 Bioassay Kit provides a easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. TGR5 receptor agonist) based activation of GLP-1R GPCR activity via detection of intracellular cAMP.
User Manuals & Protocols
70-417 cAMP Hunter CHO-K1 GPBAR1 Bioassay Kit User Manual REV0
View DocumentDatasheets
95-0049Y2x Datasheet
View Document