cAMP Hunter™ CHO-K1 GPBAR1 Bioassay Kit

Background Information:

The cAMP Hunter™ CHO-K1 GPBAR1 Bioassay Kit provides a robust and highly sensitive functional, cell-based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. TGR5 receptor agonist) based activation of GPBAR1 GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPBAR1, a naturally Gs coupled GPCR. Upon activation of GPBAR1, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Bile Acid

Sub Family:

Class A

Protein Name:

TGR5

Protein Aliases:

hGPCR19|Membrane-type receptor for bile acids|M-BAR|hBG37|BG37

Accession Number:

NM_001077194.1

UniProt Number:

Q8TDU6

Gene Name:

GPBAR1

Gene ID:

151306

Gene Aliases:

BG37|GPCR|TGR5|M-BAR|GPCR19|GPR131|MGC40597

Target Species:

Human

Cell Background:

CHO-K1