cAMP Hunter™ CHO-K1 EDG1 Gi Cell Line

The cAMP Hunter™ CHO-K1 EDG1 Gi Cell Line measures EDG1 (GPCR) activity via increased levels of intracellular cAMP. This cell line ships as two vials of cryopreserved cells.

Catalog #: 95-0142C2 Size: 2 vials

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Biological Information
Background Information:	The cAMP Hunter™ EDG1 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. sphingosine 1-phosphate) based activation of EDG1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type EDG1 , a naturally Gi/Go coupled GPCR. Upon activation of EDG1 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells.
Target Class:	GPCR
Family:	Lysophospholipid (S1P) Receptors
Sub Family:	Class A
Coupling:	Gi/Go
Accession Number:	NM_001400
Target Name:	EDG1
Target Aliases:	Sphingosine 1-phosphate receptor 1, CD363, CHEDG1, D1S3362, ECGF1, EDG-1, S1PR1, S1P1
Target Species:	Human
Cell Background:	CHO-K1

Usage
Product Type:	Stable Cell Lines
Application:	Drug Discovery & Development
Storage Conditions:	Store in vapor phase of liquid nitrogen.
Usage Disclaimer:	These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information
Assay Type:	Functional
Assay Measures:	cAMP Detection
Detection Method:	Chemiluminescence

Additional Information
Brand:	cAMP Hunter™