Biological Information | |
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Background Information: | The cAMP Hunter™ CXCR1 (IL8RA) Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CXCL8/IL8) based activation of CXCR1 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CXCR1 , a naturally Gi/Go coupled GPCR. Upon activation of CXCR1 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. |
Target Class: | GPCR |
Family: | Chemokine Receptors |
Sub Family: | Class A |
Coupling: | Gi/Go |
Accession Number: | NM_000634 |
Target Name: | CXCR1 (IL8RA) |
Target Aliases: | Chemokine (C-X-C motif) receptor 1, C-C, C-C-CKR-1, CD128, CD181, CDw128a, CKR-1, CMKAR1, IL8R1, IL8RA, IL8RBA, CXCR1, IL8RA, Chemokine |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | Stable Cell Lines |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | cAMP Detection |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | cAMP Hunter™ |
cAMP Hunter™ CHO-K1 CXCR1 (IL8RA) Gi Cell Line
The cAMP Hunter™ CHO-K1 CXCR1 (IL8RA) Gi Cell Line measures CXCR1 (GPCR) activity via increased levels of intracellular cAMP. This cell line ships as two vials of cryopreserved cells.
User Manuals & Protocols
70-233 cAMP Hunter Gs and Gi Cell Lines REV10 User Manual
View DocumentDatasheets
95-0073C2 Datasheet
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