cAMP Hunter™ CHO-K1 CALCRL-RAMP1 Gs Cell Line

The cAMP Hunter™ CHO-K1 CALCRL-RAMP1 Gs Cell Line measures CALCRL (GPCR) activity via increased levels of intracellular cAMP. This cell line ships as two vials of cryopreserved cells.

Catalog #: 95-0164C2 Size: 2 vials
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Biological Information
Background Information:
The cAMP Hunter™ CALCRL-RAMP1 Gs cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. β-CGRP) based activation of CALCRL GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type CALCRL , a naturally Gs coupled GPCR, alongside modulator protein RAMP1. Upon activation of CALCRL , the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This stable modified cell line is provided as two vials of cryopreserved cells. This product expresses CALCRL alongside the receptor modifying protein RAMP1, which increases activity of the CALCRL receptor
Target Class:
GPCR
Family:
Calcitonin Receptor
Sub Family:
Class B
Coupling:
Gs
Accession Number:
AY389506
Target Name:
CALCRL-RAMP1
Target Aliases:
CRT, CT-R, CTR, CTR1, Calcitonin receptor-like
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage Disclaimer:
These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Functional
Assay Measures:
cAMP Detection
Detection Method:
Chemiluminescence
Additional Information
Brand:
cAMP Hunter™

User Manuals & Protocols

70-233 cAMP Hunter Gs and Gi Cell Lines REV10 User Manual

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Datasheets

95-0164C2_Datasheet

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