SPRINTer™ Human BRAF(V600E) Protein Turnover Biosensor Cell-Based Assay Kit (A375)

Background Information:

The SPRINTer™ Human BRAF(V600E) Targeted Protein Degradation Cell-Based Assay Kit (A375) utilizes the SPRINTer™ BRAF(V600E) Protein Turnover Biosensor Cell Line (A375) to monitor endogenous BRAF(V600E) protein levels and allow measurement of drug-induced targeted protein degradation (TPD). Genome engineering or editing techniques, such as CRISPR/Cas9, are used to introduce a small tag into the endogenous locus of interest. This allows for the quantitation of changes in BRAF (V600E) levels when cells are treated with therapeutic agents such as targeted protein degraders. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

Kinase

Family:

TKL

Sub Family:

Protein Ser/Thr

Protein Name:

B-Raf

Protein Aliases:

Proto-oncogene B-Raf|p94|v-Raf murine sarcoma viral oncogene homolog B1

Accession Number:

NP_00161187

UniProt Number:

P15056

Gene Name:

BRAF

Gene ID:

673

Gene Aliases:

BRAF1

Target Species:

Human

Cell Background:

A375