Ready-to-Assay Y4 Neuropeptide Y Receptor Frozen Cells

Background Information:

The Ready-to-Assay Y4 Neuropeptide Y Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. peptide YY) based activation of NPY4R GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress NPY4R alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of NPY4R , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Neuropeptide Y

Sub Family:

Class A

Protein Name:

Y4

Protein Aliases:

Pancreatic polypeptide receptor 1|PP1

Accession Number:

NM_005972

UniProt Number:

P50391

Gene Name:

NPY4R

Gene ID:

5540

Gene Aliases:

Y4|PP1

Target Species:

Human

Cell Background:

Chem-1