Ready-to-Assay V2 Vasopressin Receptor Frozen Cells

Background Information:

The Ready-to-Assay V2 Vasopressin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. vasopressin) based activation of AVPR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress AVPR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of AVPR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Vasopressin and Oxytocin

Sub Family:

Class A

Protein Name:

V2

Protein Aliases:

nephrogenic diabetes insipidus

Accession Number:

NM_000054.4; NM_001146151

UniProt Number:

P30518

Gene Name:

AVPR2

Gene ID:

554

Gene Aliases:

ADHR|DIR|DIR3|V2R

Target Species:

Human

Cell Background:

Chem-1