Ready-to-Assay V1B Vasopressin Receptor Frozen Cells

Background Information:

The Ready-to-Assay V1B Vasopressin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. vasopressin) based activation of AVPR1B GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress AVPR1B alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of AVPR1B , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Vasopressin and Oxytocin

Sub Family:

Class A

Protein Name:

V1B

Protein Aliases:

AVPR V1b|AVPR V3|Antidiuretic hormone receptor 1b|Vasopressin V3 receptor

Accession Number:

NM_000707

UniProt Number:

P47901

Gene Name:

AVPR1B

Gene ID:

553

Gene Aliases:

AVPR3|VPR3

Target Species:

Human

Cell Background:

Chem-1