Ready-to-Assay V 1A Vasopressin Receptor Frozen Cells

Background Information:

The Ready-to-Assay V 1A Vasopressin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. vasopressin) based activation of AVPR1A GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress AVPR1A alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of AVPR1A , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Vasopressin and Oxytocin

Sub Family:

Class A

Protein Name:

V1A

Protein Aliases:

AVPR V1a|Antidiuretic hormone receptor 1a|Vascular/hepatic-type arginine vasopressin receptor

Accession Number:

NM_005242

UniProt Number:

P37288

Gene Name:

AVPR1A

Gene ID:

552

Gene Aliases:

AVPR1

Target Species:

Human

Cell Background:

Chem-1