Ready-to-Assay S1P5 Lysophospholipid Receptor Frozen Cells

Background Information:

The Ready-to-Assay S1P5 Lysophospholipid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. S1P) based activation of S1PR5 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress S1PR5 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of S1PR5 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Lysophospholipid (S1P)

Sub Family:

Class A

Protein Name:

S1P5

Protein Aliases:

EDG8

Accession Number:

NM_030760

UniProt Number:

Q9H228

Gene Name:

S1PR5

Gene ID:

53637

Gene Aliases:

Edg-8

Target Species:

Human

Cell Background:

Chem-5