Ready-to-Assay PTH2 Parathyroid Hormone Receptor Frozen Cells

Background Information:

The Ready-to-Assay PTH2 Parathyroid Hormone Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. parathyroid hormone) based activation of PTH2R GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTH2R alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTH2R , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Parathyroid Hormone

Sub Family:

Class B

Protein Name:

PTH2

Accession Number:

NM_005048

UniProt Number:

P49190

Gene Name:

PTH2R

Gene ID:

5746

Target Species:

Human

Cell Background:

Chem-1