Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells

Background Information:

The Ready-to-Assay PAF Platelet Activating Factor Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. PAF) based activation of PTAFR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTAFR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTAFR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Platelet-Activating Factor

Sub Family:

Class A

Protein Name:

PAF

Protein Aliases:

PAFr

Accession Number:

NM_000952

UniProt Number:

P25105

Gene Name:

PTAFR

Gene ID:

5724

Target Species:

Human

Cell Background:

Chem-1