Ready-to-Assay PAC1-long Receptor Frozen Cells

Background Information:

The Ready-to-Assay PAC1-long Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. PACAP) based activation of ADCYAP1R1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress ADCYAP1R1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADCYAP1R1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

VIP and PACAP

Sub Family:

Class B

Protein Name:

PAC1 (PACAP)

Protein Aliases:

PACAP-R-1|PACAP-R1|PACAP receptor 1

Accession Number:

AY366498; NM_001118

UniProt Number:

P41586

Gene Name:

ADCYAP1R1

Gene ID:

117

Gene Aliases:

PAC1|PACAPR|PAC1R

Target Species:

Human

Cell Background:

Chem-1