Ready-to-Assay OXGR1 alpha-Ketoglutarate Receptor Frozen Cells

Background Information:

The Ready-to-Assay OXGR1 α-Ketoglutarate Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. α-ketoglutarate) based activation of OXGR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress OXGR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of OXGR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Oxoglutarate

Sub Family:

Class A

Protein Name:

OXGR1 (GPR99 )

Protein Aliases:

2-oxoglutarate receptor 1|alpha-ketoglutarate receptor 1

Accession Number:

NM_080818.3

UniProt Number:

Q96P68

Gene Name:

OXGR1

Gene ID:

27199

Gene Aliases:

P2RY15|P2Y15|aKGR

Target Species:

Human

Cell Background:

Chem-1