Ready-to-Assay NK3 Tachykinin Receptor Frozen Cells

Background Information:

The Ready-to-Assay NK3 Tachykinin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. tachykinins) based activation of TACR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress TACR3 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of TACR3 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Tachykinin

Sub Family:

Class A

Protein Name:

NK3

Protein Aliases:

neurokinin beta receptor

Accession Number:

NM_001059

UniProt Number:

P29371

Gene Name:

TACR3

Gene ID:

6870

Gene Aliases:

NK3R|TAC3R

Target Species:

Human

Cell Background:

Chem-1