Ready-to-Assay MRGPRX1 Receptor Frozen Cells

Background Information:

The Ready-to-Assay MRGPRX1 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. BAM22) based activation of MRGPRX1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress MRGPRX1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of MRGPRX1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Protein Name:

MRGX1 (MRGPRX1)

Accession Number:

NM_147199

UniProt Number:

Q96LB2

Gene Name:

MRGPRX1

Gene ID:

259249

Gene Aliases:

MRGX1|SNSR3|SNSR4

Target Species:

Human

Cell Background:

Chem-1