Ready-to-Assay kappa Opioid Receptor Frozen Cells

Background Information:

The Ready-to-Assay κ Opioid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. DADLE, DPDPE) based activation of OPRK1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress OPRK1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of OPRK1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Opioid

Sub Family:

Class A

Protein Name:

kappa (KOP)

Protein Aliases:

KOR-1

Accession Number:

AF498922

UniProt Number:

P41145

Gene Name:

OPRK1

Gene ID:

4986

Gene Aliases:

KOR|OPRK

Target Species:

Human

Cell Background:

Chem-1