Ready-to-Assay GPR109A Nicotinic Acid Receptor Frozen Cells

Background Information:

The Ready-to-Assay GPR109A Nicotinic Acid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. nicotinic acid) based activation of HCAR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress HCAR2 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of HCAR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Hydroxycarboxylic Acid

Sub Family:

Class A

Protein Name:

GPR109A

Protein Aliases:

niacin receptor 1|HCA2

Accession Number:

NM_177551.3

UniProt Number:

Q8TDS4

Gene Name:

HCAR2

Gene ID:

338442

Gene Aliases:

HCA2|HM74A|PUMAG|Puma-g|NIACR1

Target Species:

Human

Cell Background:

Chem-4