Biological Information

Background Information:

The Ready-to-Assay GLP-1 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. GLP-1) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress GLP1R alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of GLP1R , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Protein Name:

GLP-1

Protein Aliases:

GLP-1-R|GLP-1R

Accession Number:

NM_002062

UniProt Number:

P43220

Gene Name:

GLP1R

Gene ID:

2740

Target Species:

Human

Cell Background:

HEK293

Usage

Product Type:

Frozen Cells

Application:

Drug Discovery & Development

Storage Conditions:

Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.

Usage disclaimer:

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Assay Information

Assay Type:

Cell Based

Assay Measures:

Calcium Flux

Detection Method:

Fluorescence

Clinical Relevance

Therapeutic Area:

Metabolic Diseases

Additional Information

Brand:

ChemiSCREEN™