Biological Information
Background Information:
The Ready-to-Assay GLP-1 Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. GLP-1) based activation of GLP1R GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress GLP1R alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of GLP1R , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.
Target Class:
GPCR
Family:
Glucagon
Sub Family:
Class B
Protein Name:
GLP-1
Protein Aliases:
GLP-1-R|GLP-1R
Accession Number:
NM_002062
UniProt Number:
P43220
Gene Name:
GLP1R
Gene ID:
2740
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Frozen Cells
Application:
Drug Discovery & Development
Storage Conditions:
Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
ChemiSCREEN™