Ready-to-Assay FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells

Background Information:

The Ready-to-Assay FFA3/GPR41 Free Fatty Acid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. free fatty acids) based activation of FFAR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress FFAR3 alongside exogenous levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of FFAR3 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Free Fatty Acid

Sub Family:

Class A

Protein Name:

FFA3 (GPR41)

Accession Number:

NM_005304

UniProt Number:

O14843

Gene Name:

FFAR3

Gene ID:

2865

Gene Aliases:

FFA3R

Target Species:

Human

Cell Background:

Chem-4