Ready-to-Assay CXCR3 Chemokine Receptor Frozen Cells

Background Information:

The Ready-to-Assay CXCR3 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CXCL11/I-TAC) based activation of CXCR3 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CXCR3 alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of CXCR3 , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

CXCR3

Protein Aliases:

CXCR-3|CKR-L2|G protein-coupled receptor 9|Interferon-inducible protein 10 receptor|IP-10 receptor|CD antigen CD183

Accession Number:

NM_001504.1

UniProt Number:

P49682

Gene Name:

CXCR3

Gene ID:

2833

Gene Aliases:

CKR-L2|CMKAR3|IP10-R|MigR|CD183

Target Species:

Human

Cell Background:

HEK293