Ready-to-Assay CCR10 Chemokine Receptor Frozen Cells

Background Information:

The Ready-to-Assay CCR10 Chemokine Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CCL2, MCP-1) based activation of CCR10 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CCR10 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCR10 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Protein Name:

CCR10

Protein Aliases:

CC-CKR-10|CCR-10|G-protein coupled receptor 2

Accession Number:

AF215981

UniProt Number:

P46092

Gene Name:

CCR10

Gene ID:

2826

Target Species:

Human

Cell Background:

Chem-1