Ready-to-Assay CCK1 Cholesystokinin Receptor Frozen Cells

Background Information:

The Ready-to-Assay CCK1 Cholecystokinin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. cholecystokinin) based activation of CCKAR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CCKAR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CCKAR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Cholecystokinin

Sub Family:

Class A

Protein Name:

CCK1 (CCKA)

Protein Aliases:

CCK-AR|Cholecystokinin-1 receptor|CCK1-R

Accession Number:

NM_000730

UniProt Number:

P32238

Gene Name:

CCKAR

Gene ID:

886

Target Species:

Human

Cell Background:

Chem-1