Ready-to-Assay alpha2B Adrenergic Receptor Frozen Cells

Background Information:

The Ready-to-Assay α2B Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. [ligand]) based activation of ADRA2B GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress ADRA2B alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBrite cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of ADRA2B , the resulting calcium activity can be assayed in either detection mode. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Adrenoceptor

Sub Family:

Class A

Protein Name:

alpha2B

Protein Aliases:

Alpha-2B adrenoreceptor|Alpha-2B adrenoceptor|Alpha-2BAR

Accession Number:

NM_000682

UniProt Number:

P18089

Gene Name:

ADRA2B

Gene ID:

151

Gene Aliases:

ADRA2L1|ADRA2RL1

Target Species:

Human

Cell Background:

HEK293