Biological Information
Background Information:
The Ready-to-Assay α1A Adrenergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA1A GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress ADRA1A alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADRA1A , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.
Target Class:
GPCR
Family:
Adrenoceptor
Sub Family:
Class A
Protein Name:
alpha1A
Protein Aliases:
Alpha-1A adrenoceptor|Alpha-1C adrenergic receptor|Alpha-adrenergic receptor 1c
Accession Number:
NM_000680
UniProt Number:
P35348
Gene Name:
ADRA1A
Gene ID:
148
Gene Aliases:
ADRA1L1|ADRA1C
Target Species:
Human
Cell Background:
Chem-1
Usage
Product Type:
Frozen Cells
Application:
Drug Discovery & Development
Storage Conditions:
Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.
Usage disclaimer:
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Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Additional Information
Brand:
ChemiSCREEN™