PrecisION™ Recombinant hnAChR α3/β4 Ion Channel Membrane Preparation

Background Information:

The PrecisION™ Recombinant hnAChR α3/β4 membrane prep is a crude membrane prep with high levels of hnAChR α3/β4 for use in studying binding affinity of drug candidates (small molecules or biologics) targeting ion channels. This membrane prep enables assessment of ligand binding affinity via detection of levels of radioactivity. It is derived from our proprietary stable recombinant cell lines to ensure high levels of hnAChR α3/β4 surface expression. PrecisION™ membrane preps can be used to screen for ligand binding affinities at hnAChR α3/β4 using a competitive radio-labeled ligand approach. Increased binding affinities will lead to higher radioactive signal. Membrane preps contain 1mL of 2mg/mL frozen membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression.

Target Class:

Ion Channel

Family:

Acetylcholine (Nicotinic)

Sub Family:

Voltage-Gated

Protein Name:

nAChR

Protein Aliases:

acetylcholine receptor, nicotinic, alpha 3 (neuronal)

Accession Number:

M37981, NM_000750

UniProt Number:

P32297

Gene Name:

CHRNA3

Gene ID:

1136

Target Species:

Human

Cell Background:

HEK293