PathHunter® Human HTR2C (5-HT2C) β-Arrestin Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human HTR2C (5-HT2C) β-Arrestin Cell-Based Assay Kit (U2OS) utilizes the Human HTR2C (5-HT2C) β-Arrestin cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand (e.g., serotonin) based activation of HTR2C (5-HT2C) GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged HTR2C (5-HT2C) target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Serotonin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

5-HT2C

Protein Aliases:

5-HT2C|5-HTR2C|5-hydroxytryptamine receptor 1C|5-HT-1C|5-HT1C|Serotonin receptor 2C

Accession Number:

NM_000868.2

UniProt Number:

P28335

Gene Name:

HTR2C

Gene ID:

3358

Gene Aliases:

5-HT2C|5HTR2C

Target Species:

Human

Cell Background:

U2OS