PathHunter® Jurkat SIRPa Signaling Bioassay Kit

Background Information:

The PathHunter® SIRPa Signaling Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. Engagement of the receptor by its ligand (e.g. CD-47 or agonist antibodies) results in activation of the SIRPα-ED fusion protein, which leads to the recruitment of SHP-1-EA, forcing complementation of the EFC components to create an active β-gal enzyme. This active enzyme hydrolyzes substrate to create a chemiluminescence signal as a measure of receptor activity. The bioassay kit contains all reagents needed for the assay including pre-qualified cryopreserved cells, detection reagents, cell plating reagent, positive control agonist, and assay plates.

Target Class:

Checkpoint Receptor

Family:

IgSF

Sub Family:

Immune Inhibitory Receptor

Protein Name:

SIRPA

Protein Aliases:

SHPS-1|Brain Ig-like molecule with tyrosine-based activation motifs|Bit|CD172 antigen-like family member A|Inhibitory receptor SHPS-1|Macrophage fusion receptor|MyD-1 antigen|Signal-regulatory protein alpha-1|Sirp-alpha-1|Signal-regulatory protein alpha-2|Sirp-alpha-2|Signal-regulatory protein alpha-3|Sirp-alpha-3|p84|CD antigen CD172a

Accession Number:

NM_001040022.1

UniProt Number:

P78324

Gene Name:

SIRPA

Gene ID:

140885

Gene Aliases:

BIT|MFR|MYD1|PTPNS1|SHPS1|SIRP

Target Species:

Human

Cell Background:

Jurkat