PathHunter® Human SIRP alpha Signaling Cell-Based Assay Kit (Jurkat)

Background Information:

The PathHunter® Human SIRP alpha Signaling Cell-Based Assay Kit (Jurkat) utilizes the SIRPa Checkpoint Signaling cell line. The stable engineered cell line is for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables the assessment of ligand (e.g., CD47) based activation of SIRPa receptors via detection of SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged SIRPa target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of a SH2 domain protein to the phosphorylated receptor tail leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Checkpoint Receptor

Family:

IgSF

Sub Family:

Immune Inhibitory Receptor

Protein Name:

SIRPA

Protein Aliases:

SHPS-1|Brain Ig-like molecule with tyrosine-based activation motifs|Bit|CD172 antigen-like family member A|Inhibitory receptor SHPS-1|Macrophage fusion receptor|MyD-1 antigen|Signal-regulatory protein alpha-1|Sirp-alpha-1|Signal-regulatory protein alpha-2|Sirp-alpha-2|Signal-regulatory protein alpha-3|Sirp-alpha-3|p84|CD antigen CD172a

Accession Number:

NM_001040022.1

UniProt Number:

P78324

Gene Name:

SIRPA

Gene ID:

140885

Gene Aliases:

BIT|MFR|MYD1|PTPNS1|SHPS1|SIRP

Target Species:

Human

Cell Background:

Jurkat