PathHunter® Human Siglec9 Internalization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human Siglec9 Internalization Cell-Based Assay Kit (U2OS) utilizes the Siglec9 Internalization Cell Line. This stable cell line is engineered to co-express the ProLink™ (PK) tagged Siglec9 receptor, and an Enzyme Acceptor (EA) tag localized to the endosomes. Ligand (e.g. Sialic Acid, anti-siglec9 antibody) binding to the receptor stimulates receptor internalization, resulting in colocalization of the receptor-PK with the EA in the endosomes, and forcing complementation of the two β galactosidase (β-gal) enzyme fragments (EA and PK). The resulting functional β-gal enzyme hydrolyzes substrate to generate a chemiluminescent signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Checkpoint Receptor

Family:

T Cell Receptor

Protein Name:

Siglec-9

Protein Aliases:

CDw329|Protein FOAP-9|CD329

Accession Number:

NM_014441.2

UniProt Number:

Q9Y336

Gene Name:

SIGLEC9

Gene ID:

27180

Gene Aliases:

CD329|CDw329|FOAP-9|siglec-9|OBBP-LIKE

Target Species:

Human

Cell Background:

U2OS