PathHunter® Human MCP-1 (CCR2) β-Arrestin Cell-Based Assay Kit (HEK 293)

Background Information:

The PathHunter® Human MCP-1 (CCR2) β-Arrestin Cell-Based Assay Kit (HEK 293) utilizes the Human CCR2 β-Arrestin cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand (e.g., CCL2/MCP-1) based activation of CCR2 GPCR activity via detection of β-arrestin recruitment. This cell line employs Enzyme Fragment Complementation (EFC) technology that combines an overexpressed PK-tagged CCR2 target receptor and an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. This product leverages a sensitive chemiluminescent detection system that delivers a wide dynamic range and robust Z’ for potency, efficacy, and rank‑order profiling across small molecules and biologics.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay.
All additional cell culture and assay reagents are available for purchase separately.

Target Class:

GPCR

Family:

Chemokine

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

CCR2

Protein Aliases:

CC-CKR-2|CCR-2|CCR2|Monocyte chemoattractant protein 1 receptor|MCP-1-R|CD antigen CD192

Accession Number:

NM_001123396

UniProt Number:

P41597

Gene Name:

CCR2

Gene ID:

729230

Gene Aliases:

CC-CKR-2|CKR2|MCP-1-R|CD192|FLJ78302

Target Species:

Human

Cell Background:

HEK293