PathHunter® Human GPR35 Activated GPCR Internalization Cell-Based Assay Kit (U2OS)

Background Information:

The PathHunter® Human GPR35 Activated GPCR Internalization Cell-Based Assay Kit (U2OS) utilizes the GPR35 Activated GPCR internalization cell line. The stable engineered cell line is designed for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. LPA, zaprinast) based internalization of GPR35 via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged GPR35. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting GPR35-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Coupling:

Gi/Go

Protein Name:

GPR35

Protein Aliases:

KYNA receptor

Accession Number:

NM_005301

UniProt Number:

Q9HC97

Gene Name:

GPR35

Gene ID:

2859

Target Species:

Human

Cell Background:

U2OS