PathHunter® Human beta Catenin (CTNNB1) Stable Cell Line Nuclear Translocation Assay (U2OS)

Background Information:

The PathHunter Human beta Catenin (CTNNB1) Stable Cell Line Nuclear Translocation Assay (U2OS) utilizes the U2OS β-Catenin Nuclear Translocation cell line. The stable engineered cell line is for use in studying drug candidates affecting NHR activation and nuclear translocation. This cell line enables assessment of ligand (e.g. Wnt ligands) based NHR activation via detection of resulting β-Catenin nuclear translocation. This product uses EFC technology. The included cell line overexpresses ED-tagged β-Catenin and nuclear localized EA. Upon activation of β-Catenin, induced nuclear translocation leads to production of the EFC signal.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

Nuclear Protein

Protein Name:

beta-Catenin

Accession Number:

NM_001904

UniProt Number:

P35222

Gene Name:

CTNNB1

Gene ID:

1499

Gene Aliases:

CTNNB|OK/SW-cl.35|PRO2286

Target Species:

Human

Cell Background:

U2OS