PathHunter® Human ADGRB1 (BAI1) β-Arrestin Cell-Based Assay Kit (CHO-K1)

Background Information:

The PathHunter® Human ADGRB1 (BAI1) β-Arrestin Cell-Based Assay Kit (CHO-K1) utilizes the Human ADGRB1 (BAI1) β-Arrestin orphan cell line. This engineered stable cell line is optimized for GPCR pharmacology and mechanism-of-action (MOA) studies, enabling quantitative assessment of β-arrestin recruitment following receptor activation for GPCR drug discovery and development programs. This cell line enables the assessment of ligand based activation of ADGRB1 (BAI1) GPCR activity via detection of β-arrestin recruitment. This cell line utilizes Enzyme Fragment Complementation (EFC) technology, which combines an overexpressed PK-tagged ADGRB1 (BAI1) target receptor with an EA-tagged β-Arrestin 2 to generate a chemiluminescent signal proportional to receptor activation. Please note that this cell line's target receptor is considered an orphan, and orphan receptors typically do not have established control ligands.
This kit includes a stable engineered cell line (supplied as two vials of cryopreserved cells); cell line specific reagents for cell thawing, propagation, detachment, cryopreservation, and cell plating; and a 10‑plate detection kit required to run the assay. Assay plates and positive control for use in the assays can be purchased separately. For continued use of the cell line, additional quantities of cell culture and assay reagents are available for purchase separately.
Eurofins DiscoverX optimized Detection Reagents are required for optimal assay performance and must be used with these cell lines in accordance with the cell line license agreed to upon purchase of this cell line assay kit.

Target Class:

GPCR

Sub Family:

Adhesion

Protein Name:

ADGRB1 (BAI1)

Protein Aliases:

Vstat40; Vasculostatin-120|Vstat120]

Accession Number:

NM_001702.2

UniProt Number:

O14514

Gene Name:

ADGRB1

Gene ID:

575

Gene Aliases:

BAI1

Target Species:

Human

Cell Background:

CHO-K1