PathHunter® eXpress XBP1 Nuclear Translocation Assay

Background Information:

The PathHunter® eXpress XBP1 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the IRE1/XBP1 pathway. This kit assesses ligand (e.g. thapsigargin) based pathway activation via detection of resulting XBP1 nuclear translocation. This product uses EFC technology. The cells included overexpress PL-tagged XBP1 and nuclear localized EA. Upon pathway activation, induced nuclear translocation of XBP1 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Nuclear Protein

Family:

Transcription factor

Protein Name:

XBP1

Protein Aliases:

Tax-responsive element-binding protein 5|TREB-5 [Cleaved into: X-box-binding protein 1, cytoplasmic form; X-box-binding protein 1, luminal form]

Accession Number:

BC012841

UniProt Number:

P17861

Gene Name:

XBP1

Gene ID:

7494

Target Species:

Human

Cell Background:

U2OS