Biological Information
Background Information:
The PathHunter® VISTA/VISTA Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand induced dimerization of VISTA/VISTA receptors. This product uses EFC technology. The cells included overexpress VISTA target protein fused to PK alongside VISTA target protein fused to EA. Upon ligand-induced dimerization of VISTA/VISTA, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.
Target Class:
Checkpoint Receptor
Family:
T Cell Receptor
Protein Name:
VISTA
Protein Aliases:
Stress-induced secreted protein-1|Sisp-1|V-set domain-containing immunoregulatory receptor|V-set immunoregulatory receptor
Accession Number:
NM_022153.1
UniProt Number:
Q9H7M9
Gene Name:
VSIR
Gene ID:
64115
Gene Aliases:
C10orf54|SISP1|VISTA|PP2135|UNQ730/PRO1412
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
eXpress Assay Kits
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover
Assay Information
Assay Type:
Cell Based
Assay Measures:
Dimerization
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
PathHunter®