PathHunter® eXpress UTR2 Total GPCR Internalization Assay

Background Information:

The PathHunter® UTR2 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. urotensin II) based internalization of UTR2 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged UTR2 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the UTR2 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Urotensin

Sub Family:

Class A

Coupling:

Gq

Protein Name:

UT (GPR14)

Protein Aliases:

G-protein coupled receptor 14|Urotensin II receptor|UR-II-R

Accession Number:

NM_018949

UniProt Number:

Q9UKP6

Gene Name:

UTS2R

Gene ID:

2837

Gene Aliases:

GPR14

Target Species:

Human

Cell Background:

U2OS