PathHunter® eXpress TORC2 Nuclear Translocation Assay

Background Information:

The PathHunter® eXpress TORC2 Nuclear Translocation Assay provides an easy-to-use cell based assay to study drug candidates affecting the CRTC/CREB pathway. This kit assesses ligand (e.g. forskolin) based pathway activation via detection of resulting TORC2 (CRTC2) nuclear translocation. This product uses EFC technology. The cells included overexpress PL-tagged TORC2 (CRTC2) and nuclear localized EA. Upon pathway activation, induced nuclear translocation of TORC2 leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Nuclear Protein

Family:

Transcription factor

Protein Name:

TORC2

Protein Aliases:

TORC-2|Transducer of CREB protein 2

Accession Number:

NM_181715

UniProt Number:

Q53ET0

Gene Name:

CRTC2

Gene ID:

200186

Gene Aliases:

TORC2

Target Species:

Human

Cell Background:

U2OS