Biological Information

Background Information:

The PathHunter® RXFP4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. relaxin-3) based activation of RXFP4 GPCR activity via detection of β-arrestin recruitment. This product uses EFC technology. The cells included overexpress PK-tagged RXFP4 and EA-tagged β-Arrestin-2. Activation of RXFP4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Relaxin Family Peptide

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

RXFP4

Protein Aliases:

G-protein coupled receptor 100|G-protein coupled receptor GPCR142|Insulin-like peptide INSL5 receptor|Relaxin family peptide receptor 4

Accession Number:

NM_181885.2

UniProt Number:

Q8TDU9

Gene Name:

RXFP4

Gene ID:

339403

Gene Aliases:

GPR100|RLN3R2

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

eXpress Assay Kits

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discover

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Additional Information

Brand:

PathHunter®