PathHunter® eXpress OPRM1 Total GPCR Internalization Assay

Background Information:

The PathHunter® OPRM1 Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. β-endorphin, met enkephalin) based internalization of OPRM1 via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged OPRM1 and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the OPRM1 receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Opioid

Sub Family:

Class A

Coupling:

Gi/Go

Protein Name:

mu (MOP)

Protein Aliases:

MOR-1|Mu opiate receptor|Mu opioid receptor|MOP|hMOP

Accession Number:

NM_000914.3

UniProt Number:

P35372

Gene Name:

OPRM1

Gene ID:

4988

Gene Aliases:

MOR1

Target Species:

Human

Cell Background:

U2OS